Good microbiological laboratory practice:

·        Always wear a laboratory coat or apron before entering a laboratory for protecting clothes from contamination or accidental discoloration by staining solution.

·        Wearing appropriate garments (e.g. gloves and shoe covers or lab shoes) when enter into laboratory.

·        Never eat, drink, apply cosmetics or lip balm, handle contact lenses or take medication in the laboratory.

·        Never wear jewelry (e.g. Watch, ring, etc) in microbiology laboratory.

·        Use aseptic techniques. Hand washing is essential after removing gloves and other personnel protective equipment, after handling hand sanitize with 70% IPA or 90% ethanol.

·        Microbiological testing should be performed and supervised by an experienced person, qualified in microbiology or equivalent. Staff should have basic training in microbiology and relevant practical experience before being allowed to perform work to pathogens.

·        Microbiology laboratories and certain support equipment (e.g. autoclaves and glassware) should be dedicated and separated from other areas, especially from seating areas.

·        Before and after each laboratory period, clean your workbench with a disinfectant like 70% IPA.

·        Keep your laboratory bench clean or everything (books, purses, paper, etc) except your laboratory equipment and notebook.

·        If a live culture is spilled, cover the area with a disinfectant such as mercuric chloride for 15 minutes and then clean it.

·        In the event of personal injury such as cut or burn, inform your instructor immediately as bacteria love open wounds.

·        Wash your hands with soap and water before and after finishing work in the laboratory.

·        Broth cultures must never be pipette with mouth and must be filled with the use of pipette aids and operated in such a way so as to avoid creating aerosol

·        Aseptic technique must be rigorously observed at all times.

·        Always keep culture tube in an upright position in rack or basket.

·        You must familiarize yourself in advance with the exercise to be performed.

·        Materials such as stain and reagent bottles, Petri plates, pipettes must be returned to their original location after used.

·        Always clean microscope stage, eyepiece, lenses and objectives, before and after use. All lenses must be wiped with the xylene or lens paper.

·        Keep the laboratory doors and windows closed when experiments are in progress. Precaution must be taken while removing the closures from shaken culture tubes and plunging of contamination loops into a flame to avoid inhalation of an infectious microbe, in other words, to avoid infection from laboratory.

·        All instruments like incubator, autoclave, hot air oven, laminar air flow, Bio safety cabinet etc internally calibrate on stable time duration.

·        Keep your lab logbook up-to-date.